University Archives

Fungal infections, particularly those caused by Candida albicans, represent a growing clinical concern due to limited antifungal treatments and their association with systemic diseases. In the oral cavity, fungal infections are increasingly relevant with the rise of artificial dental devices and biofilm-associated infections. Traditional in vitro models, such as catheter-based assays, fail to accurately represent oral environments, showing the need for more relevant studies. This project investigates the genetic factors involved in biofilm formation between Candida albicans and Streptococcus mutans, a key contributor to dental caries. Specifically, we examine the role of C. albicans cell wall and kinase-related genes in co-biofilm development. Using a mutant library, individual gene disruptions are screened to determine their impact on biofilm formation and stability. Weekly assays evaluate multiple mutants, followed by quantitative analysis of biofilm growth and structure. Identifying genes essential for co-biofilm formation may show therapeutic targets for preventing fungal-bacterial interactions associated with oral disease. This research aims to improve our understanding of fungal contributions to dental pathology and support the development of more effective antifungal strategies.