In Silico annotation of the genome of novel bacteriophage Kady

Cunmulaj, Jasmina, John Sherwood, Joshua Thomson, and Jacob Kagey

Kady is a novel bacteriophage isolated from soil samples as part of the first half of the HHMI SEA-Phages project. The research that is part of this project is targeted to help understand the genetic makeup of the gene sequences within the bacteriophages and add more annotated genomes to the growing databases, as this will help with the growing number of bacterial strains that cannot be controlled with common antibacterial chemicals anymore.  The research discussed in this poster was the annotation of the genome of novel bacteriophage Kady, using In Silico, or an entirely computer based method. To do this the isolated bacteriophage had It’s DNA sequenced and then the auto-annotation systems, GLIMMER and GeneMark, were used to predict genes in this novel DNA sequence. These gene predictions were then annotated using In Silico methods. In total, three tRNA’s and 47 genes were predicted, including two that were not called by auto annotation, as well as not including the two predicted to be false positives. As a result, this annotation will help future researchers better understand bacteriophage genomes, It will be especially helpful when trying to understand bacteriophages that can infect Mycobacterium Tuberculosis, as that bacteria is in the same family as Mycobacterium Smegmatis, the bacterial strain Kady was isolated with.