The Annotation of Genes 17 through 60 of bacteriophage KADY With the Programs DNA Master, Phamerator, and Starterator

Patel, Payal, Victoria Torres, Katherine Yacco, Joshua Thomson, and Jacob Kagey

Bacteriophage are viruses that infect and kill bacteria by inserting their genetic material into the genomes of bacteria. They use the bacterium's reproductive mechanisms to replicate. When there is a large accumulation of new phage the overwhelming pressure will cause the cell to burst, releasing the new bacteriophage to spread. They can be used in what is called “phage therapy,” the use of bacteriophage to attack and eliminate harmful bacteria that infect humans and livestock. The overall goal of annotating mycobacteriophage KADY, was to analyze its abilities and similarities with other bacteriophages to suggest further host bacterium. Previously, KADY was isolated from an enriched soil sample from Warren, MI  through a series of experimental methods. Mycobacterium smegmatis mc2155 was the isolation host. The isolated DNA from KADY was sequencing using the Illumina Sequencing method at the Pittsburgh Bacteriophage Institute. In this study, we annotated the 50898 bp DNA sequence from KADY with the programs DNA Master, Phamerator, and Starterator. We describe here the complete DNA sequence of the KADY genome. In total we changed two genes from the start codons that DNA Master had predicted. Many of the annotated genes do not have predicted function. However, the integrase protein was identified as Gene 34. Genes 23 and 24 were classified as the frameshift, which occur in every bacteriophage genome.